Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/zt

doi:10.3969/j.issn.1001-7216.2015.03.010

Abstract

Abstract: Specific molecular markers for detecting the alleles of Pi2/Pi9/Pizt, designated as Pi2SNP, Pi9SNP and PiztPA, respectively, have been successfully developed through sequence comparison method. These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus, which facilitate the molecular markerassistant selection and the development of pyramiding cultivars combined with other allele R genes. Additionally, 101 main cultivars and breeding parents collected from different riceplanting areas have been diagnosed with these three specific markers, and the results showed that only 2 out of 101 cultivars carried Pizt, but neither Pi2 nor Pi9 band had been detected. So far, it indicated that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Pizt. These results had provided important information for the application of Pi2/Pi9/Pizt in the blast resistance breeding programs.

Key words: rice, blast, resistance gene, molecular marker, MAS, Pi2/9 locus

摘要： 通过对已克隆的抗稻瘟病基因Pi2、Pi9以及Pizt 进行序列比对，寻找各自特异的核苷酸差异，成功开发了基于PCR技术以及电泳检测技术的 Pi2/Pi9/Pizt 以及Pizt 的基因特异性分子标记，能有效地将 Pi2/Pi9/Pizt 与该位点上的其他抗性等位基因及感病等位基因区分开，这为分子标记辅助育种及抗病基因聚合提供有效的分子标记。用 Pi2/Pi9/Pizt 基因特异性分子标记对来自全国各稻区的共101份水稻品种和育种亲本进行分子检测，结果发现，除2个品种检测到Pizt带型之外，大部分水稻品种不携带这3个抗性基因，这为有目的地开展品种的抗性改良提供了重要的参考信息。

关键词: 水稻, 稻瘟病, 抗性基因, 分子标记, 分子标记辅助选择育种, Pi2/9 位点

CLC Number:

HUA Lixia1,2,#, WANG Wenjuan1,2,# , CHEN Shen1,2, WANG Congying1,2, ZENG Liexian1,YANG Jianyuan1, ZHU Xiaoyuan1, SU Jing1,2,*. Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/zt[J]. Chinese Journal of Rice Science, 2015, 29(3): 305-310.

华丽霞1,2, #,汪文娟1,2,#,陈深1，2,汪聪颖1,2,曾烈先1,杨健源1,朱小源1,苏菁1,2,*. 抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发[J]. 中国水稻科学, 2015, 29(3): 305-310.

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